SYNTHESIS, X-RAY STRUCTURE AND DNA-BINDING OF COBALT(II), NICKEL(II), ZINC(II) AND COPPER(II) COMPLEXES WITH THE NON-STEROIDAL ANTI-INFLAMMATORY DRUG MELOXICAM
Tahereh Hosseinzadeh Sanatkar and Hassan Hadadzadeh
Isfahan University of Technology, Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran
Abstract:
Nowadays, non-steroidal anti-inflammatory drugs (NSAIDs) are an important class of medicines that have antipyretic, analgesic and anti-inflammatory properties. The coordination of NSAIDs to transition metal ions can be enhanced the anti-inflammatory activity and reduced gastro intestinal (GI) toxicity compared to the bare drugs. In this study, four new mononuclear complexes, trans-[MII(Hmel)2(EtOH)2] (where M = Co (1); Ni (2); Zn (3); Cu(4), and H2mel = 4-hydroxy-2-methyl-N-(5-methyl-2-thiazolyl)-2H-1,2-benzothiazine-3-carboxami de-1,1dioxide) were synthesized and characterized by elemental analysis and IR, 1H-NMR, and electronic absorption spectroscopies. The crystal structures of all complexes were determined by single-crystal X-ray diffraction. The interaction of the complexes with DNA has been investigated by electronic absorption, fluorescence titration, and cyclic voltammetry. In addition, CD spectroscopy and DNA cleavage experiments have been used to investigate the interaction of the complexes with DNA. The binding mode of the complexes to DNA is electrostatic. An in vitro cell cytotoxicity of the complexes on human breast adenocarcinoma (MCF7) cell line was also studied by an MTT assay.
Keywords: Meloxicam, Drug-DNA interaction and transition metal complexes.